Protein Synthesis Patterns | Zendy

Estela Sánchez de Jiménez | Zendy; Raúl Aguilar | Zendy

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Protein Synthesis Patterns

Author(s) -

Estela Sánchez de Jiménez,

Raúl Aguilar

Publication year - 1984

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.75.1.231

Subject(s) - scutellum , imbibition , messenger rna , protein biosynthesis , amino acid , translation (biology) , biology , gel electrophoresis , biochemistry , embryo , microbiology and biotechnology , chemistry , germination , botany , gene

The proteins synthesized during the first hours of seed imbibition were studied in axes and scutellum of maize embryos separately. Increase in fresh weight was followed in the embryonic axes through the germination period. Pulse labeling experiments with (14)C-amino acids were carried out at two stages of development: 0 to 6 and 18 to 24 hours in the presence and absence of alpha-amanitin. The proteins were analyzed by two-dimensional gel electrophoresis and fluorography. Results showed a major pattern of proteins common to both tissues, axes and scutellum (;house keeping' proteins), besides the specific proteins synthesized by each tissue. In the axes, the changes in proteins observed between the periods of 0 to 6 and 18 to 24 hours of development seem to be due both to newly synthesized mRNA as well as to delayed translation of stored mRNA species.

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