Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L | Zendy

Bertrand Hirel | Zendy; Claire Weatherley | Zendy; Claude Crétin | Zendy; Catherine Bergounioux | Zendy; Pierre Gadal | Zendy

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Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L

Author(s) -

Bertrand Hirel,

Claire Weatherley,

Claude Crétin,

Catherine Bergounioux,

Pierre Gadal

Publication year - 1984

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.74.2.448

Subject(s) - nicotiana tabacum , glutamine synthetase , protein subunit , biochemistry , gel electrophoresis , biology , polyacrylamide gel electrophoresis , glutamine , sodium dodecyl sulfate , microbiology and biotechnology , enzyme , gene , amino acid

Chloroplastic glutamine synthetase from tobacco leaves (Nicotiana tabacum L. var Xanthi) was purified to homogeneity. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography, a single subunit was identified with a molecular weight of 45,000 daltons. However the native protein seems to be composed of four different subunits which can be separated by isoelectrofocusing. It is suggested that different genes with eventual posttranslational and/or posttranscriptional modifications may control the synthesis of the chloroplastic glutamine synthetase.

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