Open AccessVacuolar Localization of Ethylene-Induced Chitinase in Bean Leaves
Author(s) -
Thomas Böller,
Urs Vögeli
Publication year - 1984
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.74.2.442
Subject(s) - chitinase , phaseolus , vacuole , protoplast , ethylene , intracellular , biology , enzyme , botany , biochemistry , chemistry , cytoplasm , catalysis
The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular chitinase activity.
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