Open AccessOxygen Fixation into Hydroxyproline in Etiolated Maize Seedlings
Author(s) -
George J. Fritz,
Dean D. Fetterolf,
Richard A. Yost,
Brian D. Andresen
Publication year - 1983
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.73.3.860
Subject(s) - etiolation , hydroxyproline , chemistry , oxygen , shoot , molecular mass , mass spectrometry , botany , biochemistry , chromatography , biology , organic chemistry , enzyme
Etiolated maize (Zea mays L.) seedlings were grown in the dark for 5 days in an atmosphere enriched with 10.0 atom% (18)O(2). Hydroxyproline was isolated from root and shoot tissues, purified, and methylated. It was not possible to determine (18)O incorporation into hydroxyproline by conventional mass spectrometry because the final product was not sufficiently pure. The final product was analyzed successfully by tandem mass spectrometry. The (18)O content of the hydroxyl oxygen atom was 10 +/- 0.7 atom%. This result demonstrates that the hydroxyl oxygen atom in hydroxyproline was derived exclusively from molecular oxygen.