13C Nuclear Magnetic Resonance Studies of Crassulacean Acid Metabolism in Intact Leaves of Kalanchoë tubiflora | Zendy

Mark A. Stidham | Zendy; Donald E. Moreland | Zendy; James N. Siedow | Zendy

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¹³C Nuclear Magnetic Resonance Studies of Crassulacean Acid Metabolism in Intact Leaves of Kalanchoë tubiflora

Author(s) -

Mark A. Stidham,

Donald E. Moreland,

James N. Siedow

Publication year - 1983

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.73.2.517

Subject(s) - crassulacean acid metabolism , nuclear magnetic resonance , botany , photosynthesis , biology , chemistry , physics

(13)C nuclear magnetic resonance spectroscopy of intact leaves of Kalanchoë tubiflora was used to observe Crassulacean acid metabolism in vivo. (13)C signals from C-4 of malate were observed after overnight exposure of leaves to (13)CO(2). Illumination of the labeled leaves resulted in a gradual decrease in the malate signals. After a period of darkness in normal air, (13)C signals were detected in all four carbons of malate in the previously labeled leaves. The (13)C nuclear magnetic resonance spectrum of malate in solution was pH dependent, which allowed an estimation of the vacuolar pH from the whole leaf spectrum. The pH was 4.0 following a 14-hour dark period, but rose to greater than 6.0 after 6 hours of illumination.

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