Autoanalytical Procedure for the Determination of Allantoin and Allantoic Acid in Soybean Tissue | Zendy

Peter van Berkum | Zendy; Charles Sloger | Zendy

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Autoanalytical Procedure for the Determination of Allantoin and Allantoic Acid in Soybean Tissue

Author(s) -

Peter van Berkum,

Charles Sloger

Publication year - 1983

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.73.2.511

Subject(s) - allantoin , phenylhydrazine , glyoxylic acid , glycine , chemistry , chromatography , hydrolysis , biochemistry , acid hydrolysis , nuclear chemistry , amino acid , organic chemistry

Ureide analyses of soybean (Glycine max L.) tissues were accomplished with a modified and simplified automated analysis used to determine allantoin concentration in rat urine. The length of the circuit and flow rates of the solutions were reduced, and NaOH was used for color development at room temperature. Keto-acids did not significantly interfere with the determinations of ureides except for glyoxylic acid in extracts of fresh soybean tissue. The interference caused by glyoxylic acid was avoided by adding phenylhydrazine HCl to the solution of NaOH used for alkaline hydrolysis of allantoin.

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