Distribution of Calmodulin-Stimulated Ca2+ Transport into Membrane Vesicles from Green Spinach Leaves
Author(s) -
Vladimir Stosic,
Claude Penel,
Dieter Marmé,
Hubert Greppin
Publication year - 1983
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.72.4.1136
Subject(s) - spinach , vesicle , calmodulin , distribution (mathematics) , chemistry , biophysics , botany , membrane , biochemistry , biology , enzyme , mathematical analysis , mathematics
A microsomal fraction isolated from green spinach leaves exhibited a Mg(2+) and ATP-dependent (45)Ca(2+) uptake. Addition of 10 micromolar carbonyl cyanide m-chlorophenylhydrazine had no effect. The cationophore A23187 (10 micromolar) induced the release of (45)Ca(2+) accumulated by membrane vesicles. Membranes prepared from lower epidermis showed the highest Ca(2+) accumulation activity. Microsomal fractions from petiole, lamina, and midrib were less active. The stimulation by bovine brain calmodulin was about 30% for the lower epidermis, 23.5% for midrib, and below 20% for petiole and lamina.
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