Host-Pathogen Interactions | Zendy

Noboru Yamazaki | Zendy; Stephen C. Fry | Zendy; Alan G. Darvill | Zendy; Peter Albersheim | Zendy

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Host-Pathogen Interactions

Author(s) -

Noboru Yamazaki,

Stephen C. Fry,

Alan G. Darvill,

Peter Albersheim

Publication year - 1983

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.72.3.864

Subject(s) - plasmolysis , bioassay , hydrolysis , acer pseudoplatanus , cell wall , biochemistry , chemistry , leucine , pectin , starch , chromatography , biology , amino acid , genetics

A bioassay to measure the incorporation of [(14)C]leucine into acid-precipitable polymers of suspension-cultured sycamore (Acer pseudoplatanus L.) cells is described. Using this assay, cell wall fragments solubilized from sycamore cell walls by partial acid hydrolysis are shown to contain components that inhibit the incorporation of [(14)C]leucine into the acid-precipitable polymers. This inhibition was not attributable to a suppression of [(14)C]leucine uptake. The effectiveness of the wall fragments in inhibiting [(14)C]leucine incorporation was substantially relieved by plasmolysis of the cells. Fragments released from starch and citrus pectin are shown not to possess such inhibitory activities.

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