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Cell Wall Metabolism in Ripening Fruit
Author(s) -
John M. Labavitch,
L. Carl Greve
Publication year - 1983
Publication title -
plant physiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.72.3.668
Subject(s) - ripening , metabolism , cell wall , cell metabolism , biology , chemistry , microbiology and biotechnology , biochemistry , botany
A beta-1,4-xylanase has been purified from the mixture of carbohydrate-degrading enzymes found in a commercial preparation from cultures of Trichoderma viride. Purification from the desalted enzyme mixture is accomplished either by preparative isoelectric focusing or in two-column chromatographic steps. The xylanase has maximal activity at pH 5.0 and a molecular weight of approximately 13,000 daltons. The enzyme loses activity when heated to above 45 degrees C. The xylanase degrades xylans from larch and pear cell walls in an apparently endo-fashion.

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