Inhibition of Glutamine Synthetase from Pea by Tabtoxinine-β-lactam | Zendy

Mike Thomas | Zendy; Pat J. Langston-Unkefer | Zendy; Thomas F. Uchytil | Zendy; Richard D. Durbin | Zendy

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Inhibition of Glutamine Synthetase from Pea by Tabtoxinine-β-lactam

Author(s) -

Mike Thomas,

Pat J. Langston-Unkefer,

Thomas F. Uchytil,

Richard D. Durbin

Publication year - 1983

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.71.4.912

Subject(s) - glutamine synthetase , pseudomonas syringae , lactam , enzyme , glutamine , biochemistry , molar concentration , biology , chemistry , stereochemistry , amino acid , organic chemistry , gene

Tabtoxinine-beta-lactam, a hydrolytic product of tabtoxin produced by Pseudomonas syringae pv. tabaci, apparently inactivates pea seed glutamine synthetase. Inhibition of the enzyme's initial velocity is linear over a range of 0.5 to 5 millimolar tabtoxinine-beta-lactam in the presence of 10 millimolar glutamate. A method for the purification of glutamine synthetase from dried peas is presented which gives a 30% yield with a 2,000-fold increase in specific activity. A method for obtaining highly purified tabtoxinine-beta-lactam and tabtoxin in good yields is also presented. The authenticity and purity of tabtoxinine-beta-lactam and tabtoxin were verified by chromatography, biological activity, and (1)H and (13)C nuclear magnetic resonance spectroscopy.

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