Open AccessIntracellular Distribution of Free Sugars in Quiescent Cottonseed
Author(s) -
Linda L. Muller,
T. J. Jacks
Publication year - 1983
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.71.3.703
Subject(s) - stachyose , raffinose , sucrose , tetrasaccharide , cytoplasm , biochemistry , chemistry , sugar , cottonseed , fructose , biology , chromatography , food science , polysaccharide
Quiescent cottonseeds (Gossypium hirsutum L.) were fractionated non-aqueously to protein bodies and nonparticulate cytoplasm for analyses of sugar contents. Nonparticulate cytoplasm was more than 3-fold richer in total free sugars than were protein bodies, but based on the content of cytoplasm in each fraction, sugars were distributed almost equally in them. Thin-layer chromatography and high-pressure liquid chromatography revealed five sugars: sucrose, raffinose, tetrasaccharides I and II, and stachyose. Raffinose and stachyose were located mainly, and tetrasaccharide I totally, in nonparticulate cytoplasm compared to protein bodies; sucrose and tetrasaccharide II were distributed about equally in each fraction.