Open AccessCharacterization of the α-Amylases Synthesized by Aleurone Layers of Himalaya Barley in Response to Gibberellic Acid
Author(s) -
John V. Jacobsen,
T. J. V. Higgins
Publication year - 1982
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.70.6.1647
Subject(s) - aleurone , gibberellic acid , amylase , characterization (materials science) , chemistry , botany , biology , biochemistry , enzyme , materials science , germination , nanotechnology
The gibberellic acid (GA(3))-induced alpha-amylases from the aleurone layers of Himalaya barley (Hordeum vulgare L. cv Himalaya) have been purified by cycloheptaamylose-Sepharose affinity chromatography and fractionated by DEAE-cellulose chromatography. Four fractions (alpha-amylases 1-4) were obtained which fell into two groups (A and B) on the basis of a number of characteristics. Major differences in serological characteristics and in proteolytic fingerprints were found between group A (alpha-amylases 1 and 2) and group B (alpha-amylases 3 and 4). Also, the lag time for appearance of group B enzyme activity was longer than for group A, and the appearance of group B required higher GA(3) levels than group A. The components of each group behaved similarly, although differences in proteolytic fingerprints were detected.These results together with those from other studies indicate that GA(3) differentially controls the expression of two alpha-amylase genes or groups of genes giving rise to two groups of alpha-amylases with many different properties.