Isolation of Spinach Leaf Peroxisomes in 0.25 Molar Sucrose Solution by Percoll Density Gradient Centrifugation | Zendy

Zheng Liang | Zendy; Charles Yu | Zendy; Anthony H. C. Huang | Zendy

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Isolation of Spinach Leaf Peroxisomes in 0.25 Molar Sucrose Solution by Percoll Density Gradient Centrifugation

Author(s) -

Zheng Liang,

Charles Yu,

Anthony H. C. Huang

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.70.4.1210

Subject(s) - percoll , spinacia , spinach , peroxisome , centrifugation , differential centrifugation , sucrose , chromatography , density gradient , biology , microbody , chlorophyll , biochemistry , chemistry , botany , chloroplast , gene , quantum mechanics , physics

A procedure for isolating spinach (Spinacia oleracea L.) leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradient centrifugation followed by removal of the Percoll by washing and centrifugation was established. The preparation contains more than 90% peroxisomes as intact organelles with no detectable chlorophyll or cytochrome oxidase contamination. The peroxisomes are stable at 0 to 4 degrees C or 25 degrees C for at least 2 hours.

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