Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L. | Zendy

Jerry D. Cohen | Zendy

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Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L.

Author(s) -

Jerry D. Cohen

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.70.3.749

Subject(s) - glycine , chemistry , chromatography , isotope dilution , indole test , acid hydrolysis , hydrolysis , moiety , amino acid , stereochemistry , biochemistry , mass spectrometry

Indole-3-acetyl-l-aspartate (IAAsp) was isolated from seeds of Glycine max L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was shown to be the l-enantiomer by reverse phase high performance liquid chromatographic retention time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl-beta-d-glycopyranosyl isothiocyanate. Isotope dilution analysis using [(14)C]IAAsp as internal standard showed that soybean seed contained 10 mumol/kg IAAsp and this accounted for one-half of the total indoleacetic acid of the seed.

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