Isolation and Characterization of Chloroplast DNA from Chlorella ellipsoidea | Zendy

Takashi Yamada | Zendy

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Isolation and Characterization of Chloroplast DNA from Chlorella ellipsoidea

Author(s) -

Takashi Yamada

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.70.1.92

Subject(s) - hpaii , restriction enzyme , chloroplast dna , dna , biology , chloroplast , microbiology and biotechnology , isoschizomer , restriction fragment , agarose gel electrophoresis , agarose , restriction map , dna methylation , biochemistry , gene , plasmid , gene expression

A circular DNA molecule was isolated from chloroplasts of Chorella ellipsoidea. The DNA had a buoyant density of 1.695 grams per cubic centimeter (36% GC) and a contour length of 56 micrometers (175 kilobase pairs). The restriction endonuclease analysis gave the same size. Agarose gel electrophoretic patterns of chloroplast DNA digested by several restriction endonucleases were also presented. The digestion by the restriction enzymes, HpaII, MspI, SmaI, and XmaI revealed no appreciable methylation at CG sites in chloroplast DNA.

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