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A simple method was developed for the determination of resistance coefficients for ethylene diffusion in plant tissues based on the kinetic analysis of the efflux of preloaded ethane gas. Efflux curves were analyzed to obtain first-order rate constants and resistance coefficients. Resistance coefficients determined by the ethane efflux and steady-state methods were found to agree well. Employing the ethane efflux method, it was shown that over 97% of gas exchange of tomato (Lycopersicum esculentum Mill., cv. ;Ace') fruits occurs through the stem scar. The resistances to diffusion of tomato skin and stem scar were found to be 280,000 and 300 seconds per centimeter, respectively; the combined resistance of intact tomato fruits was approximately 7,800 seconds per centimeter. The ethane efflux method was employed to show that plastic shrink-wrapping of English cucumbers (Cucumis sativus L. var anglicus Bailey) increased the resistance to ethane diffusion from 1.1 x 10(3) to 23 x 10(3) seconds per centimeter.

A Simple Method for the Determination of Resistance to Gas Diffusion in Plant Organs