Open AccessLysine-Ketoglutarate Reductase Activity in Developing Maize Endosperm
Author(s) -
Paulo Arruda,
Ladaslav Sodek,
William José da Silva
Publication year - 1982
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.69.4.988
Subject(s) - endosperm , lysine , biochemistry , reductase , chemistry , enzyme , zea mays , biology , botany , agronomy , amino acid
Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, alpha-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the K(m) for lysine and alpha-ketoglutarate were 5.2 and 1.8 millimolar, respectively.
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