Glutamine Synthetase in Spinach Leaves | Zendy

Bertrand Hirel | Zendy; Catherine PerrotRechenmann | Zendy; Akira Suzuki | Zendy; Jean Vidal | Zendy; Pierre Gadal | Zendy

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Glutamine Synthetase in Spinach Leaves

Author(s) -

Bertrand Hirel,

Catherine PerrotRechenmann,

Akira Suzuki,

Jean Vidal,

Pierre Gadal

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.69.4.983

Subject(s) - spinacia , spinach , glutamine synthetase , enzyme , chloroplast , polyacrylamide gel electrophoresis , biochemistry , glutamine , biology , gel electrophoresis , amino acid , gene

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.

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