Effects of Centrifugal Force and Centrifugation Time on the Sedimentation of Plant Organelles | Zendy

Jerry Nagahashi | Zendy; Kathleen Hiraike | Zendy

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Effects of Centrifugal Force and Centrifugation Time on the Sedimentation of Plant Organelles

Author(s) -

Jerry Nagahashi,

Kathleen Hiraike

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.69.2.546

Subject(s) - centrifugation , endoplasmic reticulum , organelle , microsome , centrifugal force , sedimentation , pellet , centrifuge , mitochondrion , chemistry , membrane , differential centrifugation , vesicle , biophysics , biology , biochemistry , sediment , enzyme , rotational speed , physics , paleontology , zoology , quantum mechanics , nuclear physics

The effect of centrifugal force and length of centrifugation time on the sedimentation of plant organelles was determined for corn (Zea mays L.) root homogenates. A centrifugal force of 6000g for at least 20 minutes was necessary to pellet 90% of the mitochondrial marker (cytochrome c oxidase). This initial centrifugation step is optimal for separating mitochondria from microsomes, since cross-contamination of endoplasmic reticulum and plasma membrane vesicles with mitochondria is minimized. Centrifugal forces of 8000g or 10,000g for 20 minutes and 13,000g for 15 minutes pellet 90% of the mitochondrial marker; however, these centrifugation conditions also sediment more plasma membrane and endoplasmic reticulum.

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