Separation of Mg-Protoporphyrin IX and Mg-Protoporphyrin IX Monomethyl Ester Synthesized de novo by Developing Cucumber Etioplasts | Zendy

Thomas P. Fuesler | Zendy; Claire M. Hanamoto | Zendy; Paul A. Castelfranco | Zendy

Open Access

Separation of Mg-Protoporphyrin IX and Mg-Protoporphyrin IX Monomethyl Ester Synthesized de novo by Developing Cucumber Etioplasts

Author(s) -

Thomas P. Fuesler,

Claire M. Hanamoto,

Paul A. Castelfranco

Publication year - 1982

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.69.2.421

Subject(s) - cucumis , protoporphyrin ix , methionine , chemistry , biochemistry , methylation , protoporphyrin , chelation , in vitro , greening , biology , organic chemistry , botany , dna , amino acid , porphyrin , photodynamic therapy , ecology

High pressure liquid chromatography was used to demonstrate that chelation of Mg(2+) into protoporphyrin IX precedes methylation in isolated greening etioplasts from cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons. Mg-protoporphyrin IX synthesized in vitro from protoporphyrin IX, Mg(2+), and ATP or exogenous Mg-protoporphyrin IX could serve as substrates for the methylation step. In either case, S-adenosylmethionine was the methyl donor and could not be replaced by ATP plus methionine.

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