Localization of DNA in Mature and Young Wheat Chloroplasts Using the Fluorescent Probe 4′-6-Diamidino-2-phenylindole | Zendy

Gun Selldén | Zendy; Rachel M. Leech | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Localization of DNA in Mature and Young Wheat Chloroplasts Using the Fluorescent Probe 4′-6-Diamidino-2-phenylindole

Author(s) -

Gun Selldén,

Rachel M. Leech

Publication year - 1981

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.68.3.731

Subject(s) - chloroplast , plastid , chloroplast dna , dna , biology , dapi , fluorescence , fluorescence microscope , genetics , staining , gene , optics , physics

The spatial organization of chloroplast DNA in developing and dividing wheat chloroplasts was studied in the light microscope using the fluorescent probe 4'-6-diamidino-2-phenylindole, which binds specifically to DNA.The DNA of wheat chloroplasts was localized at the periphery of the plastid, frequently in a discrete band. No relocalization of the DNA was observed during plastid replication. This peripheral location of the DNA was shown to differ from the central random location of DNA in tobacco and spinach chloroplasts.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research