Localization of Enzymes of Ureide Biosynthesis in Peroxisomes and Microsomes of Nodules | Zendy

Joanna F. Hanks | Zendy; N. E. Tolbert | Zendy; Karel R. Schubert | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Localization of Enzymes of Ureide Biosynthesis in Peroxisomes and Microsomes of Nodules

Author(s) -

Joanna F. Hanks,

N. E. Tolbert,

Karel R. Schubert

Publication year - 1981

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.68.1.65

Subject(s) - peroxisome , allantoin , biochemistry , microsome , endoplasmic reticulum , microbody , glycine , enzyme , biology , gram , catalase , chemistry , bacteria , amino acid , gene , genetics

The intracellular location of enzymes involved in the synthesis of the ureides, allantoin and allantoic acid, was investigated in nodules of Glycine max L. Merr. Cellular organelles were separated on isopycnic sucrose density gradients. Xanthine dehydrogenase activity (270 nanomoles per min per gram fresh weight) was totally soluble, whereas approximately 15% of the total uricase and catalase activities (1 and 2000 micromoles per minute per gram fresh weight, respectively) was in the fraction containing intact peroxisomes. Allantoinase activity (680 nanomoles per minute per gram fresh weight) was associated with the microsomal fraction, which apparently originates from the endoplasmic reticulum.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research