Effects of Cyanide on Rates and Products of Fatty Acid Synthesis by Chloroplasts Isolated from Spinacia oleracea
Author(s) -
P. Grattan Roughan,
Harry Beevers
Publication year - 1981
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.67.5.926
Subject(s) - spinacia , fatty acid synthesis , chloroplast , cyanide , biochemistry , fatty acid , glyceride , coenzyme a , biology , chemistry , enzyme , organic chemistry , reductase , gene
Cyanide inhibited unesterified fatty acid synthesis but stimulated glyceride synthesis from [1-(14)C]acetate when Spinacia oleracea chloroplasts were incubated in basal media. Both unesterified fatty acid and glyceride accumulation were inhibited when chloroplasts were incubated in a diacylglycerol mode. Stimulation of chloroplast fatty acid synthesis by either exogenous coenzyme A or Triton X-100 was almost completely abolished in the presence of cyanide. Stearoyl-ACP desaturation is considered to be inhibited to a greater extent than is fatty acid synthesis de novo.
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