Photosynthesis in Fescue

Chloroplasts isolated from tall fescue, Festuca arundinacea Schreb., showed high rates of electron transport, comparable to rates observed for spinach chloroplasts.Chloroplasts were well coupled and rates of electron transport from water to methyl viologen (photosystem II and I) were increased two to five times when ADP and inorganic phosphate or methylamine (uncoupler) were added to the reaction mixture. Ratios of P:2e for photosystem II plus I were found to be near 1.2. Electron transport rates from water to p-phenylenediamine or 2,6-dichlorobenzoquinone (photosystem II) were over 300 micromoles O(2) per hour per milligram chlorophyll, while P:2e ratios were found to be over 0.5. The highest rates of electron transport were found in electron flow from diaminodurene to methyl viologen (photosystem I) and P:2e ratios remained near 0.5.Light intensity saturation curves for photosystem II and I, as well as the photosystems independently, resembled curves for spinach, with saturation of electron transport in photosystem I and photosystem II separately occurring at 35% of the available light intensity (6000 microeinsteins per square meter per second). Photosystem II and I in sequence were saturated at about half this light intensity.