S-Adenosylmethionine Decarboxylase of Corn Seedlings | Zendy

Yonezo Suzuki | Zendy; Eiji Hirasawa | Zendy

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S-Adenosylmethionine Decarboxylase of Corn Seedlings

Author(s) -

Yonezo Suzuki,

Eiji Hirasawa

Publication year - 1980

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.66.6.1091

Subject(s) - methylglyoxal , adenosylmethionine decarboxylase , etiolation , enzyme , chemistry , germination , biochemistry , carboxy lyases , enzyme assay , hydroxylamine , yield (engineering) , specific activity , polyamine , horticulture , biology , materials science , metallurgy

S-Adenosylmethionine decarboxylase (EC 4.1.1.50) has been purified 500-fold in 30% yield from the extract of etiolated corn seedlings (cv. Golden Crossbantam Bell). This preparation had a molecular weight of approximately 25,000. The K(m) value was 5 micromolar for S-adenosylmethionine. Methylglyoxal bis(guanylhydrazone), hydroxylamine, and sulfhydryl reagents (such as p-hydroxymercuriphenylsulfonate and N-ethylmaleimide) were effective inhibitors of this enzyme. Germination of corn seed was accompanied by a rapid increase in enzyme activity and maximum activity occurred in 5-day-old seedlings.

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