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A Simple Autoradiographic Technique for Studying Diffusion of Water into Seeds
Author(s) -
G. Michael Jackson,
E. VarrianoMarston
Publication year - 1980
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.65.6.1229
Subject(s) - simple (philosophy) , diffusion , biological system , chemistry , mathematics , statistical physics , biology , physics , thermodynamics , epistemology , philosophy
Visual observations on rates and modes of water penetration into black bean seeds and into wheat and sorghum kernels during conditioning were accomplished by an autoradiographic procedure that eliminates a freezing microtome and liquid and stripping film emulsions. Seeds soaked in tritiated H(2)O were hand sectioned before freeze-quenching in liquid N(2) and subsequent block autoradiography on nuclear medicine film.

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