Incorporation and Degradation of 14C and 3H-labeled Thymidine by Sugarcane Cells in Suspension Culture | Zendy

S. M. Lesley | Zendy; Andrew Maretzki | Zendy; Louis G. Nickell | Zendy

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Incorporation and Degradation of ¹⁴C and ³H-labeled Thymidine by Sugarcane Cells in Suspension Culture

Author(s) -

S. M. Lesley,

Andrew Maretzki,

Louis G. Nickell

Publication year - 1980

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.65.6.1224

Subject(s) - thymidine , suspension culture , degradation (telecommunications) , suspension (topology) , cell culture , chemistry , biology , biochemistry , dna , genetics , mathematics , computer science , telecommunications , homotopy , pure mathematics

Sugarcane cells growing in suspension culture degrade exogenous thymidine, releasing thymine. Thymine is not utilized for DNA synthesis. Thymine is rapidly catabolized to beta-aminoisobutyric acid which is found within the cell. Thymidine in the medium is used for DNA synthesis. The label of [2-(14)C]thymidine is lost as (14)CO(2), but the label of [(3)H]methylthymidine is found in the cell as [(3)H]beta-aminoisobutyric acid, some of which is used for the synthesis of other cell components. The degradation of thymidine can be partially inhibited by addition of certain substituted pyrimidines.

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