Labeling and Isolation of Plasma Membranes from Corn Leaf Protoplasts | Zendy

David S. Perlin | Zendy; Roger M. Spanswick | Zendy

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Labeling and Isolation of Plasma Membranes from Corn Leaf Protoplasts

Author(s) -

David S. Perlin,

Roger M. Spanswick

Publication year - 1980

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.65.6.1053

Subject(s) - membrane , endoplasmic reticulum , protoplast , organelle , chloroplast , golgi apparatus , f atpase , atpase , biochemistry , chemistry , plasma , biology , chromatography , biophysics , enzyme , thylakoid , physics , quantum mechanics , gene

A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [(125)I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mitochondrial markers, whereas markers for the endoplasmic reticulum and golgi indicated minimal contamination. The highly enriched plasma membrane fraction contained a Mg(2+)-dependent, K(+)-stimulated ATPase with a pH optimum near neutrality. The position of the membranes on sucrose density gradients indicates that the plasma membranes have characteristics similar to other plasma membrane fractions.

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