Partial Purification and Characterization of the mRNA for α-Amylase from Barley Aleurone Layers | Zendy

Thomas J. Mozer | Zendy

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Partial Purification and Characterization of the mRNA for α-Amylase from Barley Aleurone Layers

Author(s) -

Thomas J. Mozer

Publication year - 1980

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.65.5.834

Subject(s) - aleurone , messenger rna , amylase , biochemistry , chromatography , reticulocyte , lysis , centrifugation , sucrose , chemistry , fractionation , gel electrophoresis , hordeum vulgare , electrophoresis , biology , enzyme , botany , poaceae , gene

The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being alpha-amylase. The mRNA for alpha-amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.

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