Effect of Cold on Endogenous Fucosyl Transferase Activity in Corn Roots
Author(s) -
Douglas W. James,
Russell L. Jones
Publication year - 1980
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.65.3.563
Subject(s) - incubation , transferase , chemistry , enzyme assay , fucose , endogeny , biochemistry , stimulation , enzyme , incubation period , biology , endocrinology , glycoprotein
Incubation of excised, 1-centimeter long root tips of corn in chilled distilled H(2)O increases the activity of extractable GDP fucose: polysaccharide fucosyl transferase. Enhancement of transferase activity is optimal at 10 C and incubation at this temperature for 8 hours causes a 3- to 5-fold stimulation of activity. Transfer of roots from 10 to 30 C reverses the enhancement of enzyme activity although reciprocal transfer of roots from 30 to 10 C does not stimulate the accumulation of transferase activity.
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