A Rapid Method for the Extraction and Analysis of Abscisic Acid from Plant Tissue | Zendy

Kerry T. Hubick | Zendy; David M. Reid | Zendy

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A Rapid Method for the Extraction and Analysis of Abscisic Acid from Plant Tissue

Author(s) -

Kerry T. Hubick,

David M. Reid

Publication year - 1980

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.65.3.523

Subject(s) - abscisic acid , maceration (sewage) , cartridge , chromatography , elution , plant tissue , extraction (chemistry) , chemistry , plant hormone , gas chromatography , biology , biochemistry , botany , materials science , metallurgy , composite material , gene

A simple, economical, and rapid method for the purification of plant extracts prior to abscisic acid (ABA) analysis is described. The method makes use of silica Sep-pak prepacked cartridges. The ABA extracts are loaded on to the Sep-pak cartridges which are then washed with a series of solvents resulting in the removal of pigments and other unwanted compounds. The ABA is then eluted from the cartridge and the levels of this hormone are estimated by gas chromatography. The whole technique (from maceration of the tissue to measurement of ABA levels) takes only 2 to 3 hours per sample.

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