Phytochrome Immunoaffinity Purification | Zendy

Robert E. Hunt | Zendy; Lee H. Pratt | Zendy

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Phytochrome Immunoaffinity Purification

Author(s) -

Robert E. Hunt,

Lee H. Pratt

Publication year - 1979

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.64.2.332

Subject(s) - phytochrome , pisum , secale , affinity chromatography , avena , isoelectric focusing , chromatography , sativum , polyacrylamide gel electrophoresis , gel electrophoresis , sodium dodecyl sulfate , biology , biochemistry , chemistry , botany , enzyme , red light

We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We have also used the immunoaffinity technique to purify phytochrome from crude oat extracts, and from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.

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