An Electron Microscope Comparison of Plasma Membrane Vesicles from Meristematic and Mature Soybean Root Tissue | Zendy

Robert Berkowitz | Zendy; R. L. Travis | Zendy

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An Electron Microscope Comparison of Plasma Membrane Vesicles from Meristematic and Mature Soybean Root Tissue

Author(s) -

Robert Berkowitz,

R. L. Travis

Publication year - 1979

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.63.6.1191

Subject(s) - vesicle , phosphotungstic acid , staining , differential centrifugation , meristem , biology , membrane , stain , chemistry , taproot , electron microscope , biochemistry , differential staining , botany , genetics , physics , gene , optics , catalysis

Plasma membrane vesicles were isolated from homogenates of meristematic and mature soybean root tissue by differential sucrose gradient centrifugation. Vesicles were positively identified by the phosphotungstic acid-chromic acid procedure (PACP). The two preparations were comparable in size class distribution, mitochondrial contamination, and per cent plasma membrane vesicles present. Purity levels were estimated to be greater than 75%. The specificity of PACP was observed for a variety of cell types from both regions. Some variability in PACP staining was offset by careful modulation of the stain protocol and was found to be independent of developmental stage in subcellular fractions. Patchy or discontinuous staining, observed in both intact tissue and in subcellular fractions from both regions, was found to be a function of stain time.

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