Open AccessPhospholipid Metabolism in Plant Mitochondria
Author(s) -
Salvatore A. Sparace,
Thomas Moore
Publication year - 1979
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.63.5.963
Subject(s) - phosphatidic acid , phosphatidylglycerol , phospholipid , membrane , phosphatidylcholine , endosperm , biochemistry , mitochondrion , inner mitochondrial membrane , inner membrane , cardiolipin , chemistry , biology
Intact mitochondria from the endosperm of castor bean were isolated on linear sucrose gradients. These mitochondria were ruptured and the membranes separated on discontinuous sucrose gradients into outer membrane, intact inner membrane, and ruptured inner membrane fractions. Each membrane fraction was examined for its capacity to synthesize phosphatidylglycerol, CDP-diglyceride, phosphatidylcholine via methylation, and phosphatidic acid. The syntheses of phosphatidylglycerol, CDP-diglyceride, and phosphatidylcholine were localized exclusively in the inner mitochondrial membrane fractions while phosphatidic acid synthesis occurred in both the inner and outer mitochondrial membranes.