Open AccessAspects of 8-[14C]Benzylaminopurine Metabolism in Phaseolus vulgaris
Author(s) -
Angelo Ramina
Publication year - 1979
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.63.2.298
Subject(s) - phaseolus , riboside , xylem , apex (geometry) , ribonucleotide , biology , botany , chemistry , biochemistry , nucleotide , gene
Phaseolus vulgaris L. plants were supplied through the root with [8-(14)C]benzylaminopurine ([(14)C]BA). Collections of root, apex, and leaves were made 8 and 48 hours after labeling; ethanolic extracts of tissues were purified and subjected to thin layer chromatography on silica gel and/or cellulose powder.From [(14)C]BA at least two different metabolites originate, the BA riboside and ribotide. The balance among [(14)C]BA, the riboside, and the ribotide changed in time. The [(14)C]BA riboside was detected as the only labeled compound in the xylem sap collected from the stem. The biological function of BA riboside and ribotide is discussed.