Rapid Increase in UDP-Glucose during Early Wheat Embryo Germination | Zendy

Chi P. Cheung | Zendy; Robert J. Suhadolnik | Zendy

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Rapid Increase in UDP-Glucose during Early Wheat Embryo Germination

Author(s) -

Chi P. Cheung,

Robert J. Suhadolnik

Publication year - 1979

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.63.1.146

Subject(s) - imbibition , embryo , germination , pyrophosphate , chemistry , biology , biochemistry , enzyme , botany , microbiology and biotechnology

The cellular content of UDP-glucose in isolated wheat (Triticum aestivum L.) embryo increases 8-fold during the first 40 minutes of imbibition. An additional 3-fold increase in the amount of UDP-glucose was observed in the next 5 hours of germination. This communication also describes a unique, quantitative method to achieve a high sensitivity in a direct determination of UDP-glucose with Na [(32)P]pyrophosphate and UDP-glucose pyrophosphorylase. The sensitivity of the assay for UDP-glucose is 10 picomoles.

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