Nitrate Utilization by the Diatom Skeletonema costatum | Zendy

Juan L. Serra | Zendy; Marı́a J. Llama | Zendy; Eduardo Cadenas | Zendy

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Nitrate Utilization by the Diatom Skeletonema costatum

Author(s) -

Juan L. Serra,

Marı́a J. Llama,

Eduardo Cadenas

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.62.6.991

Subject(s) - nitrate , nitrate reductase , ammonium , nitrogen assimilation , chemistry , nitrite , diatom , nitrogen , environmental chemistry , tungstate , assimilation (phonology) , kinetics , inorganic chemistry , botany , biology , organic chemistry , linguistics , philosophy , physics , quantum mechanics

Nitrate utilization has been characterized in nitrogen-deficient cells of the marine diatom Skeletonema costatum. In order to separate nitrate uptake from nitrate reduction, nitrate reductase activity was suppressed with tungstate. Neither nitrite nor the presence of amino acids in the external medium or darkness affects nitrate uptake kinetics. Ammonium strongly inhibits carrier-mediated nitrate uptake, without affecting diffusion transfer. A model is proposed for the uptake and assimilation of nitrate in S. costatum and their regulation by ammonium ions.

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