Phosphatidylinositol Synthesis in Castor Bean Endosperm | Zendy

James C. Sexton | Zendy; Thomas Moore | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Phosphatidylinositol Synthesis in Castor Bean Endosperm

Author(s) -

James C. Sexton,

Thomas Moore

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.62.6.978

Subject(s) - endosperm , phosphatidylinositol , inositol , endoplasmic reticulum , enzyme , ricinus , biochemistry , molar concentration , diglyceride , chemistry , pi , biology , phosphorylation , organic chemistry , receptor

CDP-diglyceride:inositol transferase in endoplasmic reticulum fractions from castor bean (Ricinus communis) endosperm was partially characterized. The enzyme had a pH optimum of 8.5 and required Mn(2+) for activity. Maximal activity was at 1.5 millimolar MnCl(2). A K(m) of 0.30 mM was calculated for myo-inositol and 1.35 millimolar was estimated for CDP-dipalmitoylglyceride. Concentrations of CDP-dipalmitoylglyceride above 1.2 millimolar inhibited the enzyme. A deoxycholate concentration of 0.1% (w/v) stimulated the reaction slightly while Triton X-100 inhibited at all concentrations tested. Some incorporation of myo-inositol into phosphatidylinositol occurred in the absence of CDP-diglyceride.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research