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Distinction between Nuclear Satellite DNAs and Chloroplast DNA in Higher Plants
Author(s) -
Martin J. Pascoe,
John Ingle
Publication year - 1978
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.62.6.975
Subject(s) - chloroplast dna , chloroplast , biology , nuclear dna , cucumis , vicia faba , satellite dna , antirrhinum majus , dna , botany , phaseolus , oenothera , biochemistry , genome , gene , mitochondrial dna
Triton X-100 solubilized chloroplast DNA but not nuclear DNA from a mixture of chloroplasts and nuclei. The buoyant density of chloroplast DNA was different from that of the satellite DNA in all of the species examined (Phaseolus coccineus, Cucumis sativus, Cucumis melo, Antirrhinum majus, Vicia faba, Oenothera fruiticosa youngii). Chloroplast DNA constituted between 4.3% and 0.25% of the total leaf DNA in these species, and was present as 5 to 20 copies in each chloroplast.

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