Determination of Indole-3-acetic Acid in Douglas Fir Using a Deuterated Analog and Selected Ion Monitoring | Zendy

John L. Caruso | Zendy; Ronald G. Smith | Zendy; Lawrence M. Smith | Zendy; TsaiYing Cheng | Zendy; G. Doyle Daves | Zendy

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Determination of Indole-3-acetic Acid in Douglas Fir Using a Deuterated Analog and Selected Ion Monitoring

Author(s) -

John L. Caruso,

Ronald G. Smith,

Lawrence M. Smith,

TsaiYing Cheng,

G. Doyle Daves

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.62.6.841

Subject(s) - deuterium , shoot , indole 3 acetic acid , chemistry , acetic acid , mass spectrometry , selected ion monitoring , chromatography , ion , botany , gas chromatography–mass spectrometry , biology , auxin , organic chemistry , biochemistry , physics , quantum mechanics , gene

Indole-3-acetic acid (IAA) content in shoot tips of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) trees and seedlings was determined by combined gas chromatography-mass spectrometry using a deuterated analog (d(2)-IAA) as an internal standard and the technique of selected ion monitoring. Ratios of the peak heights of the deuterated analog internal standard to endogenous IAA revealed a slightly higher content of IAA in seedlings compared with the shoot tips collected in June. The relatively high level of IAA (2.9 micrograms per gram fresh weight) in seedlings is discussed in relation to in vitro propagation of this species.

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