Regulatory Isoenzymes of Aspartate Kinase and the Control of Lysine and Threonine Biosynthesis in Carrot Cell Suspension Culture | Zendy

H. Maelor Davies | Zendy; Benjamin J. Miflin | Zendy

Open Access

Regulatory Isoenzymes of Aspartate Kinase and the Control of Lysine and Threonine Biosynthesis in Carrot Cell Suspension Culture

Author(s) -

H. Maelor Davies,

Benjamin J. Miflin

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.62.4.536

Subject(s) - threonine , daucus carota , lysine , biochemistry , kinase , amino acid synthesis , biology , amino acid , chemistry , enzyme , serine , botany

Aspartate kinase (EC 2.7.2.4) from carrot (Daucus carota L.) cell suspension culture has been partially resolved into lysine-sensitive and threonine-sensitive components by gel filtration chromatography. The yield of lysine-sensitive aspartate kinase changed independently of the yield of the threonine-sensitive activity during the 4-week growth cycle of the culture, and this provides additional evidence for the existence of two independently regulated isoenzymes. Exogenously supplied lysine and threonine specifically inhibited the in vivo formation of lysine and threonine, respectively, from radioactive acetate.

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