Enzymes of Krebs-Henseleit Cycle in Vitis vinifera L | Zendy

Kalliopi A. Roubelakis | Zendy; W. M. Kliewer | Zendy

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Enzymes of Krebs-Henseleit Cycle in Vitis vinifera L

Author(s) -

Kalliopi A. Roubelakis,

W. M. Kliewer

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.62.3.337

Subject(s) - ornithine , carbamyl phosphate , incubation , enzyme , molar concentration , michaelis–menten kinetics , citrulline , biochemistry , chemistry , ornithine carbamoyltransferase , phosphate , vitis vinifera , incubation period , enzyme assay , arginine , biology , botany , amino acid , organic chemistry

Ornithine carbamoyltransferase (OCT) activity was detected in extracts from mature leaves, fruit, germinating seeds, and seedlings of Vitis vinifera L. Michaelis-Menten constants for OCT were 3.5 millimolar for carbamyl phosphate and 5.5 millimolar for l-ornithine. Concentrations of l-ornithine greater than 10 millimolar slightly inhibited the enzyme, whereas carbamyl phosphate at concentrations greater than the optimal (about 10 millimolar) did not affect OCT activity. l-Citrulline formation was linear with incubation period for the first 25 minutes and with increasing amounts of enzyme up to an equivalent of about 200 milligrams of fresh tissue. The optimum pH for in vitro OCT activity was between 8.4 and 8.8, and the optimum incubation temperature was 38 C.

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