Open AccessSubcellular Localization of Isocitrate Lyase in Nongreen Tissue Culture Cells
Author(s) -
Lindsey E. Hunt,
John J. Skvarla,
John S. Fletcher
Publication year - 1978
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.61.6.1010
Subject(s) - isocitrate lyase , glycine , serine , biochemistry , isocitrate dehydrogenase , lyase , enzyme , malate synthase , chemistry , biology , amino acid , glyoxylate cycle
Density gradient centrifugation and electron microscopy were used to establish that isocitrate lyase present in Rosa cv. Paul's Scarlet cells was located in the mitochondria and not other membrane fractions. The enzyme may be important in glycine and serine synthesis. A comparison between the enzymic activity of isocitrate lyase and the amount of glycine and serine synthesized during logarithmic growth indicated that the activity was great enough to account for all of the carbon entering these amino acids during that stage of growth.