Free Flow Electrophoresis of Chloroplasts | Zendy

JeanPaul Dubacq | Zendy; JeanClaude Kader | Zendy

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Free Flow Electrophoresis of Chloroplasts

Author(s) -

JeanPaul Dubacq,

JeanClaude Kader

Publication year - 1978

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.61.3.465

Subject(s) - chloroplast , spinacia , free flow electrophoresis , electrophoresis , polyacrylamide gel electrophoresis , organelle , sodium dodecyl sulfate , gel electrophoresis , biochemistry , chromatography , spinach , gel electrophoresis of proteins , biology , chemistry , enzyme , gene

Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis retained their capacity for CO(2) fixation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that this fraction was rich in stroma and lamellae proteins. Free flow electrophoresis, which separates organelles and molecules according to their surface charges, is a good technique for producing purified chloroplasts with complete physiological activities.

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