Isolation of Bacteria, Transforming Bacteria, and Bacteroids from Soybean Nodules | Zendy

Te May Ching | Zendy; Saundra Hedtke | Zendy; William W. Newcomb | Zendy

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Isolation of Bacteria, Transforming Bacteria, and Bacteroids from Soybean Nodules

Author(s) -

Te May Ching,

Saundra Hedtke,

William W. Newcomb

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.60.5.771

Subject(s) - bacteria , nodule (geology) , biochemistry , biology , ultrastructure , glycine , endoplasmic reticulum , isolation (microbiology) , sucrose , centrifugation , differential centrifugation , botany , microbiology and biotechnology , amino acid , paleontology , genetics

Postnuclei supernatant of soybean (Glycine max cv. Chippewa 64) nodule homogenate was fractionated by stepwise sucrose density gradient centrifugation into supernatant, endoplasmic reticulum and mitochondria, and three distinct bands with 1.22, 1.25, and 1.27 g/cm(3) of peak density. Based on their enzymic activities, composition of electron transport components, and ultrastructural characteristics, the lightest band appears to be the mature bacteroids; the intermediate band the transforming bacteria; and the heaviest, the bacteria. The isolation procedure separates nodule symbionts into different functional and developmental fractions, and it may be a valuable tool for studies involving development, regulation, and senescence of bacteroids in the nodule.

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