Mesophyll Cell Protoplasts of Potato | Zendy

James F. Shepard | Zendy; Roger E. Totten | Zendy

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Mesophyll Cell Protoplasts of Potato

Author(s) -

James F. Shepard,

Roger E. Totten

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.60.2.313

Subject(s) - protoplast , solanum tuberosum , callus , shoot , biology , botany , solanaceae , cell division , tissue culture , light intensity , horticulture , cell , biochemistry , gene , physics , optics , in vitro

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintained on defined media at 24 C under 500 lux lighting. Shoot bud development within p-calli was controlled by a number of factors including light, temperature, basic medium composition, nature and source of phytohormones, the continued presence of an osmoticum, low concentrations of a utilizable carbohydrate, and the developmental stage of the p-callus.

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