A Rapid, Simple Method for Nuclei Isolation from Plant Protoplasts | Zendy

K. Ohyama | Zendy; Lawrence E. Pelcher | Zendy; Dave Horn | Zendy

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A Rapid, Simple Method for Nuclei Isolation from Plant Protoplasts

Author(s) -

K. Ohyama,

Lawrence E. Pelcher,

Dave Horn

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.60.2.179

Subject(s) - protoplast , dna , centrifugation , differential centrifugation , biology , glycine , nuclear dna , biochemistry , microbiology and biotechnology , chloroplast dna , mitochondrial dna , chloroplast , plant cell , chemistry , amino acid , gene

A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.

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