Proof for the Production of Cutinase by Fusarium solani f. pisi during Penetration into Its Host, Pisum sativum
Author(s) -
Mashouf Shaykh,
Charles L. Soliday,
P. E. Kolattukudy
Publication year - 1977
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.60.1.170
Subject(s) - cutinase , sativum , pisum , fusarium solani , biology , fungus , spore germination , botany , pathogen , spore , microbiology and biotechnology , zoospore , penetration (warfare) , biochemistry , enzyme , operations research , engineering
Rabbit antibody to cutinase-I, isolated from Fusarium solani f. pisi, was conjugated to ferritin. With this ferritin-conjugated antibody it was shown that germinating spores of this fungus excreted cutinase during the penetration of the host pisum sativum. This result constitutes the most specific and strongest evidence for an enzymic penetration of a plant cuticle by a pathogen during infection.
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