Isolation of Uridine 5′-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using 32P-Pyrophosphate | Zendy

David B. Dickinson | Zendy; David Hyman | Zendy; Jorge W. Gonzales | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Isolation of Uridine 5′-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using ³²P-Pyrophosphate

Author(s) -

David B. Dickinson,

David Hyman,

Jorge W. Gonzales

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.59.6.1082

Subject(s) - pyrophosphate , uridine , glucuronic acid , chemistry , uridine triphosphate , adenosine triphosphate , biochemistry , farnesyl pyrophosphate , chromatography , adsorption , enzyme , nucleotide , organic chemistry , biosynthesis , rna , polysaccharide , gene

A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and (32)P-pyrophosphate, and the (32)P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine (32)P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research