Characterization of a Membrane Fraction Containing a b-type Cytochrome | Zendy

Algirdas J. Jesaitis | Zendy; Patricia R. Heners | Zendy; Rainer Hertel | Zendy; Winslow R. Briggs | Zendy

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Characterization of a Membrane Fraction Containing a b-type Cytochrome

Author(s) -

Algirdas J. Jesaitis,

Patricia R. Heners,

Rainer Hertel,

Winslow R. Briggs

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.59.5.941

Subject(s) - coleoptile , endoplasmic reticulum , biochemistry , golgi apparatus , differential centrifugation , biology , etiolation , cyanide , membrane , mitochondrion , microsome , chemistry , enzyme , inorganic chemistry

The various components obtained from etiolated corn (Zea mays L.) coleoptiles were fractionated by differential or sucrose gradient centrifugation. The endoplasmic reticulum, proplastids, Golgi, and mitochondria were localized by enzymic or other markers in the various fractions. A fifth fraction was also characterized. It contains glucan synthetase II activity, binding sites for N-naphthylphthalamic acid, NADH dehydrogenase activity which is both antimycin A- and cyanide-insensitive, and a b-type cytochrome. It is possible that this fraction is plasma membrane and that it may contain the blue-ultraviolet photoreceptor for phototropism in corn.

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